Hello Everyone,

Learn how galvanic current PLUS elastin restoration can start taking years off your appearance from the very first 10 minute treatment at home.

Discover the secret that over 15,000 new people are using every month!

I’ve tried a lot of skin care products over the years, but these two technologies have given me the most impressive results…

Step #1 – Use Galvanic Current:

This CLINICALLY PROVEN TECHNOLOGY has been available through Europe’s most elite spas for the last 50 years. While highly effective for age-related skin degeneration, the size and cost of the equipment made it unavailable for use outside of commercial venues.

Until now! A hand-held galvanic device was introduced in the U.S. in 2006. Sales went from 12 in February, 2007 to more than 15,000 per month today – which is no surprise when you see results like these!

Using galvanic electrical magnetic current, this amazing hand-held device stimulates skin cells, lifts and tightens the skin, erases wrinkles, reduces puffiness, increases circulation and increases absorption of the skin by up to 70%.

Get impressive results without the pain, cost or complications of cosmetic surgical procedures.

Our patented home technology allows you to treat your skin to galvanic current at a fraction of the professional spa treatment cost. AND, you can use it in the comfort of your home as often as you like for about $8 per treatment. Treatment time is about 10 minutes, and recommended use is once or twice a week using patented treatment gels.

To view a galvanic skin treatment demo on the face and body click HERE.

Important January, 2009 Update!

New research has recently revealed a major, previously hidden source of aging within each of us – an internal aging accelerator in the epidermis, which increases as we age. Present on the surface of our cells, this dangerous enzyme called age-related NADH oxidase (arNOX) is capable of generating skin damaging free radicals around the clock that can also destroy collagen and elastin and diminish production of these critical proteins.

Individuals that have high arNOX activity in their skin typically look 7 years OLDER than their chronological age, while individuals who have low arNOX activity typically look 7 years YOUNGER.

Our galvanic skin treatment gels now contain a patented technology that targets this previously unknown INTERNAL source of aging – completely inhibiting arNOX activity in the skin.

This technology has been shown in clinical trials performed in conjunction with Purdue and Stanford to eliminate harmful arNOX free radical damage in the skin.

Download the Purdue Study HERE.

Download the Stanford Study HERE.

Download the Pilot Study of the relationship between arNOX levels and skin aging HERE.

The Pharmaceutical giant – GlaxoSmithKline – recently invested $700 Million to acquire a 10% interest in the future development of this scientific breakthrough in skin aging!

Currently, this technology is ONLY available in our galvanic skin treatment gels. However, beginning October, 2009 this revolutionary arNOX inhibitor will be available in our daily internal supplement line – providing arNOX inhibition throughout the body!

Learn more about this patented arNOX inhibitor HERE.

Step #2 – Restore Skin Elastin:

Our patented elastin serum uses a proprietary technology to restore elastin production to levels equal to an 18-25 year old. This serum is unlike anything available in stores and is the only product available that contains  a patented molecule shown to return skin elastin production to 20 year old levels.

This clinically-proven molecule has been shown to work on 99.99% of the population in UCLA clinical trials!

Skin elastin levels will be similar to that of 20 year old skin after 30-60 days of use. Skin will resume its “snap back” quality of young, smooth skin.

Learn more about this elastin technology in our Elastin Restoration blog.

While either of these technologies can be used individually with great results, the combination of these 2 treatments (galvanic current PLUS elastin restoration) has shown dramatic improvements in the skin, and typically erases years off your appearance – starting with your very first treatment.

For best results, we recommend using our daily skin care face wash with activated Vitamin C, spray toner and moisturizer – which further improves skin texture, reduces wrinkles and evens out skin discoloration.

Learn more about daily skin care tips in our Daily Skincare blog.

We offer these patented technologies to our clients at 30% off retail prices.

Please contact us for more info or to place an order.

To see more about these latest innovations in aging please watch these media clips HERE.

Before And After Treatment Photos:

Consistent Use Of Our Galvanic Current PLUS Elastin Serum

Just One 10 Minute Half Face Treatment With Our Galvanic Current

Just One 10 Minute Half Face Treatment With Our Galvanic Current

Using Our Galvanic Current

Use Galvanic Current To Reduce Cellulite! Treatment After 4 Months - 3 Times Per Week - 10 Minutes Per Treatment

Who else wants to discover the insider secrets for taking years off your appearance and achieving the most vibrant health and beauty genetically possible?

Coming soon…

Yes – I Want A Younger Future!
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I respect your privacy. I will NEVER share, rent or sell your info. As a member of my private BIO SPA group, you will be given access to the Younger Future Report and occasional BIO SPA updates about the latest in anti-aging and beauty secrets. You can un-subscribe at any time.

Get our FREE 4 part video series The Younger Future Report. This step-by-step guide cuts through the hype and will show you the simple secrets about how to look years younger and achieve vibrant wellness and beauty.

Video #1 – ANTI-AGING CONCEPTS:

• Understanding The Concept of Genetic Potential

• Four Factors That Effect Every Health And Beauty Goal You Have

Video #2 – FOUNDATION STEPS:

• Establishing The Foundation For Vibrant Wellness, Disease Prevention and Beauty

• Basic Steps That Make Sweeping Improvements In Your Body

Video #3 – ANTI-AGING AND BEAUTY 101:

• Simple Steps To Take Years Off Your Appearance

• Restore Beautiful Skin, Hair & Nails

• The 3 Important “Beauty Proteins”

• And Much More…

Video #4 – WELLNESS 101:

• Simple Steps You MUST Do To Realize Your Most Optimal Health & Wellness Genetically Possible

• How To Get A Bullet-Proof Immune System

• How To Improve Just About Any Area Of Your Health

• Create An Internal Environment Where Disease Cannot Thrive

BONUSES:

• The Secret Source For Learning How To Melt Body Fat By Changing Your Diet In A Unique Way

• FREE Access To HD Video Reviews Of Our Most Popular Anti-Aging & Wellness Technologies

• FREE Member Newsletter And Blog Updates On Numerous Wellness And Skin Care Topics

• Discount prices on exclusive anti-aging and nutritional products

Hello everyone,

Clinically-proven molecule shown to work on 99.99% of the population!

Skin elastin levels will be similar to that of 20 year old skin after 60 days.

Elastin gives the skin resiliency and the ability to stretch and snap back into shape, much like a rubber band. In fact, many skin specialists indicate that elastin is perhaps the most important skin grader in determining a persons apparent age. When we are young, our skin has high levels of elastin – on average, 14-18 percent. As we age, our elastin levels naturally decrease. Clinical trials confirm that in patients 40 years and older elastin levels drop to as low as .1% and are typically in the 9% range. Due to this decline, after the age of 25–30, our resilient and taut skin begins to sag and wrinkle.

Having beautiful, tight and firm skin allows one to feel good about their appearance and have a healthy self-image. Many people are looking for non-surgical ways to improve the appearance of their skin. To this end, a biochemist designed the breakthrough ingredient that is changing faces around the world.

This is a unique, patented molecule that is scientifically proven to be essential in the search for beautiful skin and the treatment of skin aging. It combats Intrinsic, or chronological, aging. As we age, our skin invariably loses the elasticity and firmness it once possessed in younger years. This molecule treats and reverses this Intrinsic Aging by increasing skin’s ability to produce elastin fibers.

DHT Receptor Site

This organically extracted molecule was specifically designed by a biochemist, to mimic, or simulate, the process that naturally occurs in younger skin. This molecule is a competitive inhibitor. This means it blocks something from taking place. When applied topically to your skin, it blocks DHT-DHT receptor binding in fibroblast skin cells, thereby allowing elastin production to continue at levels of young (18-25 year old) skin. This discovery’s efficacy has been proven in over 19 years of research and over $20 million in testing and development.

In medically published punch biopsy clinical studies, “This molecule caused (p=.001) tested individuals, between 40-72 years of age, to regain the important skin elastin fiber content of 20 year old skin“ .

- (Richard A. Strick, M.D. Clinical Prof. of Dermatology, UCLA School of Medicine).

“Independent of patients’ initial elastin fiber content, within 8 weeks of their twice daily applications, users showed an average of 100% improvement in their skin’s elastin content, and restoration of skin’s firmness and snapback quality.  The results were identical in both males and females, Caucasian and Asian skin types, and individuals from 25 to 77 years of age.”

The elastin levels will be similar to that of 20 year old skin after 30-60 days of use. Skin will resume its “snap back” quality of young, smooth skin.

This new topical was designed to mimic, or simulate, the process that naturally occurs in young skin. It is a competitive inhibitor. This means it blocks something from taking place. This unique serum, when applied topically to your skin, block what causes DHT-DHT receptor binding in skin cells allowing elastin production to continue at youthful levels – without causing production to exceed what you naturally produced in younger years. This is important, as you don’t want to cause hyper-production of elastin fibers.

The biochemist who developed this revolutionary topical has chosen to partner exclusively with one company to offer this exciting technology to the retail market. It now includes a green tea extract along with other antioxidants to further protect and nourish the skin. Despite my initial skepticism (I’m only 38 and look pretty good already) I’ve been using the product for several months now, and can confirm that it really does make a difference. My skin feels amazing, and I definitely see improved softness and tightness in my skin.

As the owner of BIO SPA, a science-based anti-aging and wellness business, I have been recommending this technology to all my clients – and it remains one of my most popular and best selling products. It’s simply one of those “no BS” products that just does what it claims (and has published studies done at UCLA to back it up)!

- Peter T. Pugliese, M.D.

I am offering this exclusive technology to my clients at 30% off current retail prices.

Please contact us for more info or to place an order.

More details on this revolutionary discovery can be found HERE.

Hello everyone,

New technology inhibits potent arNOX enzyme in skin cells, removing a destructive source of internal aging.

New research has recently revealed a major, previously hidden source of aging within each of us – an internal aging accelerator in the epidermis, which increases as we age. Present on the surface of our cells, this invisible enemy called age-related NADH oxidase (arNOX) is capable of generating skin damaging free radicals around the clock that can also destroy collagen and elastin and diminish production of these critical proteins.

Individuals that have high arNOX activity in their skin typically look 7 years OLDER than their chronological age, while individuals who have low arNOX activity typically look 7 years YOUNGER.

Our galvanic skin treatment gels now contain a patented technology that targets this previously unknown INTERNAL source of aging – completely inhibiting arNOX activity in the skin.

This technology has been shown in clinical trials performed in conjunction with Purdue and Stanford to eliminate harmful arNOX free radical damage in the skin.

Download the Purdue Study HERE.

Download the Stanford Study HERE.

Download the Pilot Study of the relationship between arNOX levels and skin aging HERE.

The Pharmaceutical giant – GlaxoSmithKline – recently invested $700 Million to acquire a 10% interest in the future development of this scientific breakthrough in skin aging!

Currently, this technology is ONLY available in our galvanic skin treatment gels.

However, beginning October, 2009 this revolutionary arNOX inhibitor will be available in our daily internal supplement line – providing arNOX inhibition throughout the body!

Learn more about our galvanic skin treatment and non-surgical face lift HERE.

Hello everyone,

How to use Galvanic Magnetic Current to reduce cellulite on your body.

Get UNBELIEVABLE RESULTS with proven Galvanic Magnetic Current.

This CLINICALLY PROVEN TECHNOLOGY has been available through Europe’s most elite spas for the last 50 years. While highly effective for age-related skin degeneration, the size and cost of the equipment made it unavailable for use outside of commercial venues.

Until now! A hand-held device was introduced in the U.S. in 2006. Sales went from 12 in February, 2007 to more than 15,000 per month today – which is no surprise when you see results like these!

Using Galvanic Magnetic Current to stimulate skin cells and break down fat, this amazing hand-held device gives you surgery-like results without the pain, cost or complications of cosmetic surgical procedures.

These are REAL, UNTOUCHED PHOTOS of a client Casey, who used Galvanic current three times a week, 10 minutes each for 4 months. She is 59!

Galvanic Current Used On The Stomach For 4 Months, 3 Times Per Week

To view a galvanic current treatment on the body click HERE and watch part 5 of the video demo.

We offer this patented technology to our clients at 30% off retail prices.

Please contact us for more info or to place an order.

Learn the secrets of daily skin care.

Coming soon…

How to prevent future damage, reduce wrinkles, improve skin texture, reduce pore size and skin discoloration.

PLUS – Tips on diet tricks and supplements that help prevent sun damage, free radical damage and elastin degradation.

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Limited Time Offer – Free Mineral Makeup Trial:

Hello Everyone,

Give yourself a microdermabrasion at home.

Our microdermabrasion cream peel delivers skin-smoothing results, equivalent to a professional microdermabrasion session, without ever leaving home. This non-abrasive formula features pumpkin enzymes that resurface and polish the skin, and bentonite clay to remove dull skin cells and toxins. An alternative to professional treatments, it delivers a smooth, fresh, healthy complexion — it’s clinical skin care without the clinic.

•  Re-surfaces and polishes for beautifully smooth skin that uniformly reflects light.

•  Provides results clinically proven to be equivalent to professional microdermabrasion treatments.

•  Convenient, safe and cost effective in-home method.

•  Smoothes and softens skin for a fresh healthy glow.

Clinicals

In a split face study, 91 percent of participants said their skin felt resurfaced after the home treatment peel, and said our treatment provided the same skin smoothing results as professional microdermabrasion.

Participants compared the results of a professional resurfacing session (performed on one-half of their face) to the results of our home treatment peel (applied to the other half). Following the treatments, the immediate feel of the skin was evaluated. Our home treatment was preferred by the majority of participants, and 97 percent said their skin was softer after using our home peel.

I am offering this exclusive technology to my clients at 30% off current retail prices.

Please contact us for more info or to place an order.

Learn how to effectively clear acne and restore a healthy complexion.

PLUS – Diet tips and tricks to encourage clear skin.

Please contact us for more info regarding our patented acne skin care products.

Results After 2 Weeks: Using Our Acne Treatment And Galvanic Current

How antioxidants affect your health, beauty and aging.

Antioxidants are found in Nature in compounds that are particularly rich in hydrogen and excess electrons. These hydrogen-rich and electron-rich compounds are usually visible to our eye as pigments of color in plants.

The richly pigmented plants prove to be the easiest way to discover and track down where real antioxidant values are to be found. Superfoods ranging from cacao to acai to goji berries to spirulina to blue-green algae to marine phytoplankton all contain extraordinarily-rich antioxidant content.

Antioxidants can be water or fat soluble. All raw, pure fats and oils are antioxidants as they contain excess electrons. That means, that not only are richly-colored foods loaded with antioxidants, but so are all raw olives, avocados, nuts, and seeds.

Antioxidants are known to stop runaway free radicals. Free radicals behave like bad drunks because they create problems in every cell they touch. Free radicals are aggressive oxygen molecules that oxidize and damage tissue. Free radical damage has been implicated in numerous symptoms ranging from skin wrinkles all the way to cancer.

According to the free radical theory, the more antioxidants you consume, the better, due to the ability of antioxidants to quench and deactivate free radicals.

Not only are antioxidants capable of quieting free radicals, some antioxidants have medicinal value. The xanthone antioxidants in the rind of the mangosteen fruit are known to fight the organisms that cause rheumatism and arthritis better than any other known botanical substance. The blue-pigment antioxidants in blue-green algae help increase the natural production of stem cells.

Vitamins A, C, and E are considered antioxidants. Vitamin A is a metabolite of beta-carotene, a red pigment commonly found in plants (e.g. goji berries, carrots, etc.). Vitamin C is a well-known antioxidant with a tremendous volume of research behind it. We believe that natural vitamin C sources (Camu berry) instead of simply ascorbic acid are best. Vitamin E has undergone substantial revision in recent years as its antioxidant value varies with quality and type (tocotrienols or tocopherols). Tocotrienols are more powerful Vitamin E antioxidants than tocopherols.

Some atomic minerals, such as zinc and selenium, are considered antioxidants, but they are not capable of antioxidant action by themselves. These antioxidant minerals must work in conjunction with antioxidant enzymes in order to be effective.

By David Wolfe.

List of antioxidants:

Carotenoids

Plants are constantly being bombarded by sunlight and ultraviolet rays. Since they can’t just go inside or erect a parasol when they’ve had enough sun, they produce strong antioxidants to protect their cells against cell damage from ultraviolet radiation and other environmental carcinogens. It is a potent group of antioxidants, and having a daily intake of them is an important disease-preventing measure. Several hundred of these antioxidants are known to exist, and the most potent and beneficial ones are these six:
Alpha-carotene
Beta-carotene
Cryptoxanthin
Lycopene
Lutein
Zeaxanthin

See how you can measure these important Carotenoids in our Measure Antioxidant Levels blog.

Flavonoids

Flavonoids are antioxidant phytochemicals that form the water-soluble colors of vegetables, fruits, grains, leaves, and bark. Flavonoids come in many forms, and different plants contain different concentrations of them. Some flavonoids may have up to fifty times more antioxidant activity than vitamins C and E. Impressively, flavonoids from red grapes are more than a thousand times more powerful than vitamin E in inhibiting oxidation of human LDL cholesterol. A steady intake of flavonoids can do a lot for your health.
Catechins
Reserveratrol
Proanthocyanidins

Isoflavones

Isoflavones are found in soybeans and other legumes. In the body, they are converted to phytoestrogens (plant estrogens), compounds that may help inhibit the growth of hormone-dependent cancers and other cancers. In addition, they lower total cholesterol levels and protect against heart disease.
Genistein
Daidzein

Vitamins

Vitamin A
Vitamin C
Vitamin E

Minerals

All minerals are antioxidants and must be acquired through the diet, because none of them are produced by the body. Minerals are exceedingly important for the proper functioning of the body and the assimilation of vitamins. Selenium and zinc are the two most powerful mineral antioxidants.
Selenium
Zinc

Allium vegetables

The genus allium encompasses over 500 plants, but the best antioxidants in this group are garlic, onions, shallots, and leeks. These vegetables contain flavonoids, vitamin C, selenium, and sulfur compounds that have potent cancer-fighting properties. In addition, they may help prevent heart attacks stroke by lowering cholesterol and blood pressure and preventing blood clots. They benefit the liver by activating detoxification enzyme systems, and may be helpful in preventing allergies and asthma. These vegetables retain their antioxidant properties even when cooked, and odorless garlic caps are available.

Bilberry

This potent antioxidant, which is an herb, is also called European blueberry. It contains anthocyanides, which keep capillaries strong, protect against cataracts and night blindness, and improve circulation. As well as having anti-carcinogenic effects, it inhibits the growth of harmful bacteria and acts as an anti-inflammatory. Bilberry supplements are widely available, either as pills or in liquid form.

Coenzyme-Q10

This antioxidant is also known as ubiquinone, since it is present in every living cell. It provides the cells with energy to effectively carry out their functions. Without this antioxidant, our cells would simply not work. The levels of Q10 fall with increasing age, a fact which may have impact on the diseases and illnesses we associate with age. Infection, stress, and poor eating habits can also affect the body’s ability to produce sufficient amounts of this necessary enzyme. Q10 shares many of its antioxidant properties with vitamin E. It has been shown to increase energy, improve heart function, help reverse gum disease, and strengthen the immune system. It may also slow the deterioration rate in Parkinson’s disease, and is the only known substance to have that effect. Coenzyme Q10 has many other positive effects and is a most valuable antioxidant ally in disease prevention and anti-aging. Coenzyme Q10 is found in meat, dairy products, eggs, cereals and vegetables. Storage, processing and cooking significantly reduces the Q10 content of these foods, so supplementing your diet with additional Q10 is a good idea.

Cruciferous vegetables

Broccoli, Brussels sprouts, cabbage, cale etc. are very rich in antioxidants such as vitamin C and other flavonoids, and also phytochemicals called indoles and sulforaphane. Indoles inactivate estrogens that promote the growth of tumors, particularly in the female breast. Sulforaphane stimulates cells to produce cancer-fighting enzymes. This combination of antioxidants makes cruciferous vegetables potent cancer-preventers. Cruciferous vegetables may not be among the most popular foods, but many of their beneficial antioxidant effects may be obtained through supplements.

Ginkgo Biloba

Ginkgo Biloba is a potent antioxidant herb which is best known for improving circulation. It helps increase the blood supply to the brain, the heart, and all other body parts. It strengthens the mental processes and the ability to concentrate, as well as ease muscle pain and improve male potency. For this last reason, Ginkgo Biloba is sometimes known as “nature’s Viagra”. Interestingly, this antioxidant may improve perception and social functioning in victims of Alzheimer’s disease. Like many other antioxidants, it protects cells from free radicals and may therefore prevent cancer and slow the aging process. Macular degeneration of the eyes may be prevented and treated with Ginkgo Biloba, which also has been shown to work as an antidepressant. Ginkgo Biloba is widely available as a supplement.

Glutathione

This antioxidant is produced in the liver from amino acids. It protects cells throughout the body, and may help prevent cancer, especially of the liver. Glutathione is beneficial as an immune system booster, a detoxifier of heavy metals and drugs, and may also protect against radiation poisoning and negative effects of smoking and alcohol abuse. It also functions as an anti-inflammatory treatment of allergies and arthritis. Glutathione is found in vegetables and fruit, but cooking will significantly reduce its potency. Taking it as a supplement is a good idea.

Lipoic acid

This antioxidant is produced naturally by the body. It is a unique defender against free radicals, and is sometimes called the universal antioxidant. Many other internally produced antioxidants have specific jobs, whereas lipoic acid enhances the activities of other antioxidants in the body, as well as being an excellent antioxidant on its own account. Lipoic acid may, for instance, temporarily fill in for vitamins C or E if their levels are low. Because of its ability to pass through the blood-brain barrier, it can reverse the negative effects caused by a stroke. Lipoic acid helps normalize blood sugar levels and can prevent serious complications from diabetes. Our bodies’ production of lipoic acid decreases with advancing age, so it is a very beneficial supplement for those over age forty.

Melatonin

This antioxidant hormone is produced by the brain’s pineal gland during sleep and helps maintain the body’s natural biorhythm. Melatonin may also help retard the aging process, especially by preventing the oxidative damage to brain cells that are associated with Alzheimer’s and other conditions. Cluster headaches may be alleviated by melatonin, and it has the ability to boost the immune system’s ability to stop tumors from spreading. Melatonin is found in tomatoes and other vegetables, and is available as a supplement. As we age, the body produces less and less melatonin, so for a lot of people over forty, a melatonin supplement is a good idea.

Superoxide dismutase

This antioxidant is an enzyme which is particularly powerful with skin tissue, revitalizing cells and reducing the rate of cell destruction. Superoxide dismutase helps the body use essential zinc, copper and manganese. Good sources of this antioxidant are barley grass, cabbage, wheatgrass and broccoli. As we age, our bodies produces less of this beneficial antioxidant, so supplementation could be an important factor in reducing wrinkles and retarding the aging process.

Hello everyone,

Now you can accurately measure these important protective molecules in your body in just 5 minutes with a non-invasive laser technology.

To learn more about antioxidants please visit our Power of Antioxidants blog.

Please contact us to arrange a FREE test at one of thousands of locations worldwide.

Technology:

A new technology using Raman Spectroscopy is showing up in more and more holistic physicians’ offices, medical spas, and health and fitness facilities.  Over 6 million people worldwide have received this important measurement.  Clearly, there is something novel and compelling about this technology.  At the core, however, the question remains:

Is it valid?

Does the measurement it obtains really “mean anything” in the hard light of the peer-reviewed medical literature?

A quick review of the important points relating to this technology:

This technology is the only method to obtain any reasonable approximation of total body antioxidant status by a laser scan and readout of a “skin carotenoid score.”

This measurement has been clinically proven to be more accurate than blood testing for carotenoid antioxidants.

Simultaneously, there is only one company that has an antioxidant supplement – should a deficiency be indicated – guaranteed to perform in any specific manner. In their case, the guarantee is to raise the skin carotenoid score – something which is globally associated with improving antioxidant status.

No other company can offer a performance guarantee in the consumers own body, since no other company has a measurement tool to track their product’s absorption.

Science:

A review of the original patent for this technology is instructive.  Here follow relevant excerpts from the United States Patent Application 6,205,354 – granted March 20, 2001, and awarded to Werner Gellermann and colleagues at the University of Utah.  All quotes are directly excerpted from the patent.  It should be noted that the patent was granted as specified, meaning that the US Patent Office was satisfied with the claims and the supporting documentation.

Specifically, the patent claims (Claim #9):

“A method for non-invasively determining the antioxidant status in skin tissue.”

From the Abstract:

“A method and apparatus are provided for the determination of levels of carotenoids and similar chemical compounds in biological tissue such as living skin.  The method and apparatus provide a noninvasive, rapid, accurate, and safe determination of carotenoid levels which in turn can provide diagnostic information regarding cancer risk, or can be a marker for conditions where carotenoids or other antioxidant compounds may provide diagnostic information.  Such early diagnostic information allows for the possibility of preventative intervention.”

So, how does it work?

Also from the Abstract:

“In this technique, laser light is directed upon the area of tissue which is of interest.  A small fraction of the scattered light is scattered inelastically, producing the carotenoid Raman signal that is at a different frequency than the incident laser light, and the Raman signal is collected, filtered, and measured.  The resulting Raman signal can be analyzed such that the background fluorescence signal is subtracted and the results displayed and compared with known calibration standards.”

History of the Raman detection method:

Sir C.V. Raman

Interestingly, the first hint of the discovery of the “Raman effect” occurred when Sir Chandrashekhar Venkata Raman was on a sea voyage and became fascinated with the extremely deep blue color of the Mediterranean Sea and attempted to discover the reason.  He rejected the theory that it was caused by a “reflection from the sky” and showed in 1922 that it was the scattering of light by water molecules which accounted for the “color shift.”   For the discovery of the “inelastic scattering of light,” he was awarded the Nobel Prize in Physics in 1930.  His discovery became extremely relevant with the advent of lasers and was soon applied to use in spectroscopic applications for identifications of compounds -  using the same effect he had witnessed so many decades ago on the opalescent Mediterranean Sea.